Tsubakuro is a member of Nyūdōunsai Sanada's pack, the Sanada Clan.
He is red-brown and white Great Dane with black splotches around his eyes.
He is very loyal to Sanada Nyudounsai and begins to shed tears over his Lord when he gets carried away for treatment.
Ginga Densetsu Weed: Orion
He sees Kawasemi return to say that assassins from Kurohabaki Masamune's pack. Follows him to confront the clan saying they aren't interested in joining.
Tsubakuro, Kawasemi and Sanada Nyūdōunsai save Tesshin from Kurohabaki Masamune's pack. After they've escaped Tesshin tries to convince them to help Unsai refuses and the great dane leaves with his leader. Kurohabaki Masamune injures him later during the last battle.
Ginga: The Last Wars
Tsubakuro has returned to Nagano with other the Sanada ninja dogs. Reika and the puppies enter into their territory to tell them that Ou was attacked Monsoon when they agree to help.
Sometime later, a flood causes a wounded Ken to be washed into a river and the Ou army go to the location to find and save him. They each keep a watchful eye on the raging river, as Kawasemi dives in to find him.
After Ken is saved, Tsubakuro follows the others in the battle against Monsoon. He watches Lord Unsai get injured by the bears, when the enemy flees and humans arrive. Unsai collapses from his wounds and Tsubakuro watches with others and with tears in his eyes begging for his Lord not to die as he's carried away for treatment.
Tsubakuro follows Kawasemi and Pet Dog Squad to finish off the bears this time with the help of Hidetoshi and the hunters all the bears are defeated. Tsubakuro and Kawasemi begin to notice a helicopter knowing Monsoon won't come back as along as humans are around.
He witnesses Akame losing consciousness due to his injures but thanks to Daisuke and Hidetoshi, he is quickly revived and watches as the kishu is taken away for treatment.
With Gin's return he tells them the passing of their friends they all sleep together for the night. When morning comes Tsubakuro hears Kyoshiro chew Orion out for sending his friends away and then watches the fight by Orion and Kyoshiro even though the kishu lost. He saw Orion crying in tears saying that he stayed behind for the sake of everyone because the Ohu Army needs a true leader.
He and the others look for Jerome and Orion when they fall out the cliff. Then, they find Jerome and Orion safe and sound near the river.
See more pictures in Tsubakuro (GDWO)/Gallery